Construct: shRNA TRCN0000435056

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207355.2-1357s21c1
DNA Barcode:
TGTATCTTCTCAAGATCTATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC339010 (339010)
Gene Description:
POTE ankyrin domain family, member B
Transcript:
RefSeq NM_207355.2 (NON-CURRENT)
Match location:
Position 1357 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435056)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTATCTTCTCAAGATCTATC
Hairpin Sequence:
5'-CCGG-TGTATCTTCTCAAGATCTATC-CTCGAG-GATAGATCTTGAGAAGATACA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTATCTTCTCAAGATCTATCCTCGAGGATAGATCTTGAGAAGATACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTATCTTCTCAAGATCTATCCTCGAGGATAGATCTTGAGAAGATACA-3'

Other clones with same target sequence:

(none)