Construct: shRNA TRCN0000435063

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004188.4-937s21c1
DNA Barcode:
CATGAAGAAGCACACCTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GFI1B (8328)
Gene Description:
growth factor independent 1B transcriptional repressor
Transcript:
RefSeq NM_004188.4 (NON-CURRENT)
Match location:
Position 937 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435063)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGAAGAAGCACACCTACAT
Hairpin Sequence:
5'-CCGG-CATGAAGAAGCACACCTACAT-CTCGAG-ATGTAGGTGTGCTTCTTCATG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGAAGAAGCACACCTACATCTCGAGATGTAGGTGTGCTTCTTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGAAGAAGCACACCTACATCTCGAGATGTAGGTGTGCTTCTTCATG-3'

Other clones with same target sequence:

(none)