Construct: shRNA TRCN0000435086

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014489.2-1476s21c1
DNA Barcode:
TTCATAGGACTAATGTATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PGAP2 (27315)
Gene Description:
post-GPI attachment to proteins 2
Transcript:
RefSeq NM_014489.2 (NON-CURRENT)
Match location:
Position 1476 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435086)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCATAGGACTAATGTATTTC
Hairpin Sequence:
5'-CCGG-TTCATAGGACTAATGTATTTC-CTCGAG-GAAATACATTAGTCCTATGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCATAGGACTAATGTATTTCCTCGAGGAAATACATTAGTCCTATGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCATAGGACTAATGTATTTCCTCGAGGAAATACATTAGTCCTATGAA-3'

Other clones with same target sequence:

(none)