Construct: shRNA TRCN0000435212

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_145238.3-3587s21c1
DNA Barcode:
CTTACGAGATTAGAGTTAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZSCAN20 (7579)
Gene Description:
zinc finger and SCAN domain containing 20
Transcript:
RefSeq NM_145238.3 (NON-CURRENT)
Match location:
Position 3587 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435212)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTACGAGATTAGAGTTAAAT
Hairpin Sequence:
5'-CCGG-CTTACGAGATTAGAGTTAAAT-CTCGAG-ATTTAACTCTAATCTCGTAAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTACGAGATTAGAGTTAAATCTCGAGATTTAACTCTAATCTCGTAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTACGAGATTAGAGTTAAATCTCGAGATTTAACTCTAATCTCGTAAG-3'

Other clones with same target sequence:

(none)