Construct: shRNA TRCN0000435231

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000764.2-1578s21c1
DNA Barcode:
CGGACCAAGATGCCCTACATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYP2A7 (1549)
Gene Description:
cytochrome P450 family 2 subfamily A member 7
Transcript:
RefSeq NM_000764.2 (CURRENT)
Match location:
Position 1578 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435231)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGACCAAGATGCCCTACATG
Hairpin Sequence:
5'-CCGG-CGGACCAAGATGCCCTACATG-CTCGAG-CATGTAGGGCATCTTGGTCCG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGACCAAGATGCCCTACATGCTCGAGCATGTAGGGCATCTTGGTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGACCAAGATGCCCTACATGCTCGAGCATGTAGGGCATCTTGGTCCG-3'

Other clones with same target sequence:

(none)