Construct: shRNA TRCN0000435267

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001393.2-615s21c1
DNA Barcode:
CAAAGAGAACCTACCAATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ECM2 (1842)
Gene Description:
extracellular matrix protein 2
Transcript:
RefSeq NM_001393.2 (NON-CURRENT)
Match location:
Position 615 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435267)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGAGAACCTACCAATTTA
Hairpin Sequence:
5'-CCGG-CAAAGAGAACCTACCAATTTA-CTCGAG-TAAATTGGTAGGTTCTCTTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGAGAACCTACCAATTTACTCGAGTAAATTGGTAGGTTCTCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGAGAACCTACCAATTTACTCGAGTAAATTGGTAGGTTCTCTTTG-3'

Other clones with same target sequence:

(none)