Construct: shRNA TRCN0000435282

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080660.3-1229s21c1
DNA Barcode:
ATTTGTTCACATCGTACATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZC3HAV1L (92092)
Gene Description:
zinc finger CCCH-type containing, antiviral 1 like
Transcript:
RefSeq NM_080660.3 (NON-CURRENT)
Match location:
Position 1229 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435282)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTTGTTCACATCGTACATAA
Hairpin Sequence:
5'-CCGG-ATTTGTTCACATCGTACATAA-CTCGAG-TTATGTACGATGTGAACAAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTTGTTCACATCGTACATAACTCGAGTTATGTACGATGTGAACAAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTTGTTCACATCGTACATAACTCGAGTTATGTACGATGTGAACAAAT-3'

Other clones with same target sequence:

(none)