Construct: shRNA TRCN0000435341

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014456.3-1299s21c1
DNA Barcode:
GGAACTGGAAGTACCTCATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PDCD4 (27250)
Gene Description:
programmed cell death 4
Transcript:
RefSeq NM_014456.3 (NON-CURRENT)
Match location:
Position 1299 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435341)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAACTGGAAGTACCTCATTT
Hairpin Sequence:
5'-CCGG-GGAACTGGAAGTACCTCATTT-CTCGAG-AAATGAGGTACTTCCAGTTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAACTGGAAGTACCTCATTTCTCGAGAAATGAGGTACTTCCAGTTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAACTGGAAGTACCTCATTTCTCGAGAAATGAGGTACTTCCAGTTCC-3'

Other clones with same target sequence:

  1. TRCN0000321156