Construct: shRNA TRCN0000435407

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032145.4-2106s21c1
DNA Barcode:
TCTCATGTGTATCCAAGTTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXO30 (84085)
Gene Description:
F-box protein 30
Transcript:
RefSeq NM_032145.4 (NON-CURRENT)
Match location:
Position 2106 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435407)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTCATGTGTATCCAAGTTAA
Hairpin Sequence:
5'-CCGG-TCTCATGTGTATCCAAGTTAA-CTCGAG-TTAACTTGGATACACATGAGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTCATGTGTATCCAAGTTAACTCGAGTTAACTTGGATACACATGAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTCATGTGTATCCAAGTTAACTCGAGTTAACTTGGATACACATGAGA-3'

Other clones with same target sequence:

(none)