Construct: shRNA TRCN0000435461

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014954.3-2460s21c1
DNA Barcode:
GAACCACGTGTCAAGTGATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RPH3A (22895)
Gene Description:
rabphilin 3A
Transcript:
RefSeq NM_014954.3 (NON-CURRENT)
Match location:
Position 2460 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435461)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACCACGTGTCAAGTGATTA
Hairpin Sequence:
5'-CCGG-GAACCACGTGTCAAGTGATTA-CTCGAG-TAATCACTTGACACGTGGTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACCACGTGTCAAGTGATTACTCGAGTAATCACTTGACACGTGGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACCACGTGTCAAGTGATTACTCGAGTAATCACTTGACACGTGGTTC-3'

Other clones with same target sequence:

(none)