Construct: shRNA TRCN0000435495

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005739.3-1417s21c1
DNA Barcode:
GACGTTATCCCTGGATCTTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RASGRP1 (10125)
Gene Description:
RAS guanyl releasing protein 1
Transcript:
RefSeq NM_005739.3 (NON-CURRENT)
Match location:
Position 1417 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435495)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGTTATCCCTGGATCTTTA
Hairpin Sequence:
5'-CCGG-GACGTTATCCCTGGATCTTTA-CTCGAG-TAAAGATCCAGGGATAACGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGTTATCCCTGGATCTTTACTCGAGTAAAGATCCAGGGATAACGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGTTATCCCTGGATCTTTACTCGAGTAAAGATCCAGGGATAACGTC-3'

Other clones with same target sequence:

(none)