Construct: shRNA TRCN0000435526

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000876.2-7666s21c1
DNA Barcode:
CAAGCACCTCCAACCAAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGF2R (3482)
Gene Description:
insulin like growth factor 2 receptor
Transcript:
RefSeq NM_000876.2 (NON-CURRENT)
Match location:
Position 7666 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435526)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGCACCTCCAACCAAATAA
Hairpin Sequence:
5'-CCGG-CAAGCACCTCCAACCAAATAA-CTCGAG-TTATTTGGTTGGAGGTGCTTG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGCACCTCCAACCAAATAACTCGAGTTATTTGGTTGGAGGTGCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGCACCTCCAACCAAATAACTCGAGTTATTTGGTTGGAGGTGCTTG-3'

Other clones with same target sequence:

(none)