Construct: shRNA TRCN0000435541

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003532.2-354s21c1
DNA Barcode:
GACCATCATGCCTAAAGACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
H3C6 (8353)
Gene Description:
H3 clustered histone 6
Transcript:
RefSeq NM_003532.2 (CURRENT)
Match location:
Position 354 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435541)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCATCATGCCTAAAGACAT
Hairpin Sequence:
5'-CCGG-GACCATCATGCCTAAAGACAT-CTCGAG-ATGTCTTTAGGCATGATGGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCATCATGCCTAAAGACATCTCGAGATGTCTTTAGGCATGATGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCATCATGCCTAAAGACATCTCGAGATGTCTTTAGGCATGATGGTC-3'

Other clones with same target sequence:

(none)