Construct: shRNA TRCN0000435558

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021173.3-818s21c1
DNA Barcode:
AGTCAGACATGGACAGTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
POLD4 (57804)
Gene Description:
DNA polymerase delta 4, accessory subunit
Transcript:
RefSeq NM_021173.3 (NON-CURRENT)
Match location:
Position 818 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435558)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTCAGACATGGACAGTTGAT
Hairpin Sequence:
5'-CCGG-AGTCAGACATGGACAGTTGAT-CTCGAG-ATCAACTGTCCATGTCTGACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTCAGACATGGACAGTTGATCTCGAGATCAACTGTCCATGTCTGACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTCAGACATGGACAGTTGATCTCGAGATCAACTGTCCATGTCTGACT-3'

Other clones with same target sequence:

(none)