Construct: shRNA TRCN0000435559

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053155.2-3169s21c1
DNA Barcode:
ACGACTCTCTGACGCAGTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clmn (94040)
Gene Description:
calmin
Transcript:
RefSeq NM_053155.2 (CURRENT)
Match location:
Position 3169 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435559)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGACTCTCTGACGCAGTTTG
Hairpin Sequence:
5'-CCGG-ACGACTCTCTGACGCAGTTTG-CTCGAG-CAAACTGCGTCAGAGAGTCGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGACTCTCTGACGCAGTTTGCTCGAGCAAACTGCGTCAGAGAGTCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGACTCTCTGACGCAGTTTGCTCGAGCAAACTGCGTCAGAGAGTCGT-3'

Other clones with same target sequence:

(none)