Construct: shRNA TRCN0000435565

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020819.4-1585s21c1
DNA Barcode:
CTCAATTCATTACCTATAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FAM135A (57579)
Gene Description:
family with sequence similarity 135 member A
Transcript:
RefSeq NM_020819.4 (CURRENT)
Match location:
Position 1585 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435565)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCAATTCATTACCTATAATC
Hairpin Sequence:
5'-CCGG-CTCAATTCATTACCTATAATC-CTCGAG-GATTATAGGTAATGAATTGAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCAATTCATTACCTATAATCCTCGAGGATTATAGGTAATGAATTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCAATTCATTACCTATAATCCTCGAGGATTATAGGTAATGAATTGAG-3'

Other clones with same target sequence:

(none)