Construct: shRNA TRCN0000435606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198031.1-995s21c1
DNA Barcode:
AGGAGTCTGGACCGCTCATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tubgcp3 (259279)
Gene Description:
tubulin, gamma complex associated protein 3
Transcript:
RefSeq NM_198031.1 (CURRENT)
Match location:
Position 995 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAGTCTGGACCGCTCATTT
Hairpin Sequence:
5'-CCGG-AGGAGTCTGGACCGCTCATTT-CTCGAG-AAATGAGCGGTCCAGACTCCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAGTCTGGACCGCTCATTTCTCGAGAAATGAGCGGTCCAGACTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAGTCTGGACCGCTCATTTCTCGAGAAATGAGCGGTCCAGACTCCT-3'

Other clones with same target sequence:

(none)