Construct: shRNA TRCN0000435778

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133239.2-4505s21c1
DNA Barcode:
TGGAGTCACTGAGTAGTTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crb1 (170788)
Gene Description:
crumbs family member 1, photoreceptor morphogenesis associated
Transcript:
RefSeq NM_133239.2 (CURRENT)
Match location:
Position 4505 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435778)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGAGTCACTGAGTAGTTATG
Hairpin Sequence:
5'-CCGG-TGGAGTCACTGAGTAGTTATG-CTCGAG-CATAACTACTCAGTGACTCCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGAGTCACTGAGTAGTTATGCTCGAGCATAACTACTCAGTGACTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGAGTCACTGAGTAGTTATGCTCGAGCATAACTACTCAGTGACTCCA-3'

Other clones with same target sequence:

(none)