Construct: shRNA TRCN0000435780

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002370.2-402s21c1
DNA Barcode:
GTTCCCTTATTGATGTCAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGOH (4116)
Gene Description:
mago homolog, exon junction complex subunit
Transcript:
RefSeq NM_002370.2 (NON-CURRENT)
Match location:
Position 402 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435780)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCCCTTATTGATGTCAATC
Hairpin Sequence:
5'-CCGG-GTTCCCTTATTGATGTCAATC-CTCGAG-GATTGACATCAATAAGGGAAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCCCTTATTGATGTCAATCCTCGAGGATTGACATCAATAAGGGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCCCTTATTGATGTCAATCCTCGAGGATTGACATCAATAAGGGAAC-3'

Other clones with same target sequence:

(none)