Construct: shRNA TRCN0000435799

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002927.3-416s21c1
DNA Barcode:
AGTAGTCTATGCAGCATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGS13 (6003)
Gene Description:
regulator of G protein signaling 13
Transcript:
RefSeq NM_002927.3 (NON-CURRENT)
Match location:
Position 416 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435799)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTAGTCTATGCAGCATATTT
Hairpin Sequence:
5'-CCGG-AGTAGTCTATGCAGCATATTT-CTCGAG-AAATATGCTGCATAGACTACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTAGTCTATGCAGCATATTTCTCGAGAAATATGCTGCATAGACTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTAGTCTATGCAGCATATTTCTCGAGAAATATGCTGCATAGACTACT-3'

Other clones with same target sequence:

(none)