Construct: shRNA TRCN0000435812

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015230.2-4349s21c1
DNA Barcode:
GGAACCCGACTGTAGTATTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARAP2 (116984)
Gene Description:
ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 2
Transcript:
RefSeq NM_015230.2 (NON-CURRENT)
Match location:
Position 4349 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435812)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGAACCCGACTGTAGTATTAT
Hairpin Sequence:
5'-CCGG-GGAACCCGACTGTAGTATTAT-CTCGAG-ATAATACTACAGTCGGGTTCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGAACCCGACTGTAGTATTATCTCGAGATAATACTACAGTCGGGTTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGAACCCGACTGTAGTATTATCTCGAGATAATACTACAGTCGGGTTCC-3'

Other clones with same target sequence:

(none)