Construct: shRNA TRCN0000435824

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139130.2-1209s21c1
DNA Barcode:
TCGGGTCACCAAACGCAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Coro6 (216961)
Gene Description:
coronin 6
Transcript:
RefSeq NM_139130.2 (CURRENT)
Match location:
Position 1209 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435824)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCGGGTCACCAAACGCAACAT
Hairpin Sequence:
5'-CCGG-TCGGGTCACCAAACGCAACAT-CTCGAG-ATGTTGCGTTTGGTGACCCGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCGGGTCACCAAACGCAACATCTCGAGATGTTGCGTTTGGTGACCCGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCGGGTCACCAAACGCAACATCTCGAGATGTTGCGTTTGGTGACCCGA-3'

Other clones with same target sequence:

(none)