Construct: shRNA TRCN0000435831

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032576.4-1872s21c1
DNA Barcode:
TTGTGTGTTCATCACCTTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CYorf15B (84663)
Gene Description:
chromosome Y open reading frame 15B
Transcript:
RefSeq NM_032576.4 (NON-CURRENT)
Match location:
Position 1872 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435831)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTGTGTGTTCATCACCTTAAA
Hairpin Sequence:
5'-CCGG-TTGTGTGTTCATCACCTTAAA-CTCGAG-TTTAAGGTGATGAACACACAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTGTGTGTTCATCACCTTAAACTCGAGTTTAAGGTGATGAACACACAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTGTGTGTTCATCACCTTAAACTCGAGTTTAAGGTGATGAACACACAA-3'

Other clones with same target sequence:

(none)