Construct: shRNA TRCN0000435854

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007972.3-1831s21c1
DNA Barcode:
TCCCTTGACTTCATGGTATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
F10 (14058)
Gene Description:
coagulation factor X
Transcript:
RefSeq NM_007972.3 (NON-CURRENT)
Match location:
Position 1831 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435854)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCCCTTGACTTCATGGTATAC
Hairpin Sequence:
5'-CCGG-TCCCTTGACTTCATGGTATAC-CTCGAG-GTATACCATGAAGTCAAGGGA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCCCTTGACTTCATGGTATACCTCGAGGTATACCATGAAGTCAAGGGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCCCTTGACTTCATGGTATACCTCGAGGTATACCATGAAGTCAAGGGA-3'

Other clones with same target sequence:

(none)