Construct: shRNA TRCN0000435858

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018262.2-1289s21c1
DNA Barcode:
AGACATGCATTACCGGGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IFT122 (55764)
Gene Description:
intraflagellar transport 122
Transcript:
RefSeq NM_018262.2 (NON-CURRENT)
Match location:
Position 1289 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435858)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGACATGCATTACCGGGTAAA
Hairpin Sequence:
5'-CCGG-AGACATGCATTACCGGGTAAA-CTCGAG-TTTACCCGGTAATGCATGTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGACATGCATTACCGGGTAAACTCGAGTTTACCCGGTAATGCATGTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGACATGCATTACCGGGTAAACTCGAGTTTACCCGGTAATGCATGTCT-3'

Other clones with same target sequence:

(none)