Construct: shRNA TRCN0000435883

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006558.1-466s21c1
DNA Barcode:
GCGCCTGGTGAACCAAGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KHDRBS3 (10656)
Gene Description:
KH RNA binding domain containing, signal transduction associated 3
Transcript:
RefSeq NM_006558.1 (NON-CURRENT)
Match location:
Position 466 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435883)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCCTGGTGAACCAAGAAAT
Hairpin Sequence:
5'-CCGG-GCGCCTGGTGAACCAAGAAAT-CTCGAG-ATTTCTTGGTTCACCAGGCGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCCTGGTGAACCAAGAAATCTCGAGATTTCTTGGTTCACCAGGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCCTGGTGAACCAAGAAATCTCGAGATTTCTTGGTTCACCAGGCGC-3'

Other clones with same target sequence:

(none)