Construct: shRNA TRCN0000435958

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024575.3-779s21c1
DNA Barcode:
TTCAATCTTCAGGCTTCATTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TNFAIP8L2 (79626)
Gene Description:
TNF alpha induced protein 8 like 2
Transcript:
RefSeq NM_024575.3 (NON-CURRENT)
Match location:
Position 779 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435958)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCAATCTTCAGGCTTCATTC
Hairpin Sequence:
5'-CCGG-TTCAATCTTCAGGCTTCATTC-CTCGAG-GAATGAAGCCTGAAGATTGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCAATCTTCAGGCTTCATTCCTCGAGGAATGAAGCCTGAAGATTGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCAATCTTCAGGCTTCATTCCTCGAGGAATGAAGCCTGAAGATTGAA-3'

Other clones with same target sequence:

(none)