Construct: shRNA TRCN0000435991

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001150.2-3012s21c1
DNA Barcode:
GACGATTCTCCACCGAGTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANPEP (290)
Gene Description:
alanyl aminopeptidase, membrane
Transcript:
RefSeq NM_001150.2 (NON-CURRENT)
Match location:
Position 3012 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000435991)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGATTCTCCACCGAGTATG
Hairpin Sequence:
5'-CCGG-GACGATTCTCCACCGAGTATG-CTCGAG-CATACTCGGTGGAGAATCGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGATTCTCCACCGAGTATGCTCGAGCATACTCGGTGGAGAATCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGATTCTCCACCGAGTATGCTCGAGCATACTCGGTGGAGAATCGTC-3'

Other clones with same target sequence:

(none)