Construct: shRNA TRCN0000436010

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003048.3-1779s21c1
DNA Barcode:
ACCAACCAAAGTCAAGTATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC9A2 (6549)
Gene Description:
solute carrier family 9 member A2
Transcript:
RefSeq NM_003048.3 (NON-CURRENT)
Match location:
Position 1779 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436010)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAACCAAAGTCAAGTATTG
Hairpin Sequence:
5'-CCGG-ACCAACCAAAGTCAAGTATTG-CTCGAG-CAATACTTGACTTTGGTTGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAACCAAAGTCAAGTATTGCTCGAGCAATACTTGACTTTGGTTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAACCAAAGTCAAGTATTGCTCGAGCAATACTTGACTTTGGTTGGT-3'

Other clones with same target sequence:

(none)