Construct: shRNA TRCN0000436068

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009541.2-2747s21c1
DNA Barcode:
ACCTATACAAGTGACAATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zbtb17 (22642)
Gene Description:
zinc finger and BTB domain containing 17
Transcript:
RefSeq NM_009541.2 (CURRENT)
Match location:
Position 2747 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436068)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTATACAAGTGACAATTTA
Hairpin Sequence:
5'-CCGG-ACCTATACAAGTGACAATTTA-CTCGAG-TAAATTGTCACTTGTATAGGT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTATACAAGTGACAATTTACTCGAGTAAATTGTCACTTGTATAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTATACAAGTGACAATTTACTCGAGTAAATTGTCACTTGTATAGGT-3'

Other clones with same target sequence:

(none)