Construct: shRNA TRCN0000436087

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027218.2-385s21c1
DNA Barcode:
TGGACATTCATGCTGCTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Clec4b1 (69810)
Gene Description:
C-type lectin domain family 4, member b1
Transcript:
RefSeq NM_027218.2 (NON-CURRENT)
Match location:
Position 385 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436087)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGACATTCATGCTGCTTATT
Hairpin Sequence:
5'-CCGG-TGGACATTCATGCTGCTTATT-CTCGAG-AATAAGCAGCATGAATGTCCA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGACATTCATGCTGCTTATTCTCGAGAATAAGCAGCATGAATGTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGACATTCATGCTGCTTATTCTCGAGAATAAGCAGCATGAATGTCCA-3'

Other clones with same target sequence:

(none)