Construct: shRNA TRCN0000436149

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003108.3-1142s21c1
DNA Barcode:
TTCGACCTGAGCTTGAATTTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SOX11 (6664)
Gene Description:
SRY-box transcription factor 11
Transcript:
RefSeq NM_003108.3 (NON-CURRENT)
Match location:
Position 1142 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436149)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCGACCTGAGCTTGAATTTC
Hairpin Sequence:
5'-CCGG-TTCGACCTGAGCTTGAATTTC-CTCGAG-GAAATTCAAGCTCAGGTCGAA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCGACCTGAGCTTGAATTTCCTCGAGGAAATTCAAGCTCAGGTCGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCGACCTGAGCTTGAATTTCCTCGAGGAAATTCAAGCTCAGGTCGAA-3'

Other clones with same target sequence:

  1. TRCN0000365833