Construct: shRNA TRCN0000436159

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024515.4-1188s21c1
DNA Barcode:
GAAACAGGAACCCAGCTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WDR25 (79446)
Gene Description:
WD repeat domain 25
Transcript:
RefSeq NM_024515.4 (NON-CURRENT)
Match location:
Position 1188 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436159)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAACAGGAACCCAGCTATTT
Hairpin Sequence:
5'-CCGG-GAAACAGGAACCCAGCTATTT-CTCGAG-AAATAGCTGGGTTCCTGTTTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAACAGGAACCCAGCTATTTCTCGAGAAATAGCTGGGTTCCTGTTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAACAGGAACCCAGCTATTTCTCGAGAAATAGCTGGGTTCCTGTTTC-3'

Other clones with same target sequence:

(none)