Construct: shRNA TRCN0000436233

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152510.2-1394s21c1
DNA Barcode:
GAATCCCTCTCTAGCTGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HORMAD2 (150280)
Gene Description:
HORMA domain containing 2
Transcript:
RefSeq NM_152510.2 (NON-CURRENT)
Match location:
Position 1394 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436233)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATCCCTCTCTAGCTGTATT
Hairpin Sequence:
5'-CCGG-GAATCCCTCTCTAGCTGTATT-CTCGAG-AATACAGCTAGAGAGGGATTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATCCCTCTCTAGCTGTATTCTCGAGAATACAGCTAGAGAGGGATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATCCCTCTCTAGCTGTATTCTCGAGAATACAGCTAGAGAGGGATTC-3'

Other clones with same target sequence:

(none)