Construct: shRNA TRCN0000436234

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010391.4-510s21c1
DNA Barcode:
GATTATCACCCGACGCAAGTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
H2-Q10 (15007)
Gene Description:
histocompatibility 2, Q region locus 10
Transcript:
RefSeq NM_010391.4 (CURRENT)
Match location:
Position 510 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436234)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATTATCACCCGACGCAAGTG
Hairpin Sequence:
5'-CCGG-GATTATCACCCGACGCAAGTG-CTCGAG-CACTTGCGTCGGGTGATAATC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATTATCACCCGACGCAAGTGCTCGAGCACTTGCGTCGGGTGATAATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATTATCACCCGACGCAAGTGCTCGAGCACTTGCGTCGGGTGATAATC-3'

Other clones with same target sequence:

(none)