Construct: shRNA TRCN0000436270

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030934.4-893s21c1
DNA Barcode:
GAATAAAGGAGAGACTAAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRMT1L (81627)
Gene Description:
tRNA methyltransferase 1 like
Transcript:
RefSeq NM_030934.4 (NON-CURRENT)
Match location:
Position 893 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436270)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAATAAAGGAGAGACTAAATC
Hairpin Sequence:
5'-CCGG-GAATAAAGGAGAGACTAAATC-CTCGAG-GATTTAGTCTCTCCTTTATTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAATAAAGGAGAGACTAAATCCTCGAGGATTTAGTCTCTCCTTTATTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAATAAAGGAGAGACTAAATCCTCGAGGATTTAGTCTCTCCTTTATTC-3'

Other clones with same target sequence:

(none)