Construct: shRNA TRCN0000436291

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015059.2-1618s21c1
DNA Barcode:
GACGAATCCAAACACGAAATC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TLN2 (83660)
Gene Description:
talin 2
Transcript:
RefSeq NM_015059.2 (CURRENT)
Match location:
Position 1618 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436291)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACGAATCCAAACACGAAATC
Hairpin Sequence:
5'-CCGG-GACGAATCCAAACACGAAATC-CTCGAG-GATTTCGTGTTTGGATTCGTC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACGAATCCAAACACGAAATCCTCGAGGATTTCGTGTTTGGATTCGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACGAATCCAAACACGAAATCCTCGAGGATTTCGTGTTTGGATTCGTC-3'

Other clones with same target sequence:

(none)