Construct: shRNA TRCN0000436356

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000238.2-3320s21c1
DNA Barcode:
CTCACCTTGGACTCGCTTTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KCNH2 (3757)
Gene Description:
potassium voltage-gated channel subfamily H member 2
Transcript:
RefSeq NM_000238.2 (NON-CURRENT)
Match location:
Position 3320 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436356)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCACCTTGGACTCGCTTTCT
Hairpin Sequence:
5'-CCGG-CTCACCTTGGACTCGCTTTCT-CTCGAG-AGAAAGCGAGTCCAAGGTGAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCACCTTGGACTCGCTTTCTCTCGAGAGAAAGCGAGTCCAAGGTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCACCTTGGACTCGCTTTCTCTCGAGAGAAAGCGAGTCCAAGGTGAG-3'

Other clones with same target sequence:

(none)