Construct: shRNA TRCN0000436379

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033082.3-109s21c1
DNA Barcode:
AGAATGTCTTGCTCGTGGTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SARNP (84324)
Gene Description:
SAP domain containing ribonucleoprotein
Transcript:
RefSeq NM_033082.3 (NON-CURRENT)
Match location:
Position 109 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436379)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAATGTCTTGCTCGTGGTTT
Hairpin Sequence:
5'-CCGG-AGAATGTCTTGCTCGTGGTTT-CTCGAG-AAACCACGAGCAAGACATTCT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAATGTCTTGCTCGTGGTTTCTCGAGAAACCACGAGCAAGACATTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAATGTCTTGCTCGTGGTTTCTCGAGAAACCACGAGCAAGACATTCT-3'

Other clones with same target sequence:

(none)