Construct: shRNA TRCN0000436392

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183191.2-2632s21c1
DNA Barcode:
TAACCATCAATGAGATCTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plch1 (269437)
Gene Description:
phospholipase C, eta 1
Transcript:
RefSeq NM_183191.2 (NON-CURRENT)
Match location:
Position 2632 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436392)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAACCATCAATGAGATCTTTG
Hairpin Sequence:
5'-CCGG-TAACCATCAATGAGATCTTTG-CTCGAG-CAAAGATCTCATTGATGGTTA-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAACCATCAATGAGATCTTTGCTCGAGCAAAGATCTCATTGATGGTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAACCATCAATGAGATCTTTGCTCGAGCAAAGATCTCATTGATGGTTA-3'

Other clones with same target sequence:

(none)