Construct: shRNA TRCN0000436430

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005577.2-6260s21c1
DNA Barcode:
GTGAAGCATCAACCTACTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LPA (4018)
Gene Description:
lipoprotein(a)
Transcript:
RefSeq NM_005577.2 (NON-CURRENT)
Match location:
Position 6260 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436430)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAAGCATCAACCTACTTAG
Hairpin Sequence:
5'-CCGG-GTGAAGCATCAACCTACTTAG-CTCGAG-CTAAGTAGGTTGATGCTTCAC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAAGCATCAACCTACTTAGCTCGAGCTAAGTAGGTTGATGCTTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAAGCATCAACCTACTTAGCTCGAGCTAAGTAGGTTGATGCTTCAC-3'

Other clones with same target sequence:

(none)