Construct: shRNA TRCN0000436553

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152546.2-967s21c1
DNA Barcode:
AGTTGTCATTCTTCAGTTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRFBP1 (153443)
Gene Description:
serum response factor binding protein 1
Transcript:
RefSeq NM_152546.2 (NON-CURRENT)
Match location:
Position 967 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436553)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTGTCATTCTTCAGTTAAG
Hairpin Sequence:
5'-CCGG-AGTTGTCATTCTTCAGTTAAG-CTCGAG-CTTAACTGAAGAATGACAACT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTGTCATTCTTCAGTTAAGCTCGAGCTTAACTGAAGAATGACAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTGTCATTCTTCAGTTAAGCTCGAGCTTAACTGAAGAATGACAACT-3'

Other clones with same target sequence:

(none)