Construct: shRNA TRCN0000436570

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001023563.3-2389s21c1
DNA Barcode:
GGTCTGGAAACTTACTCAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF805 (390980)
Gene Description:
zinc finger protein 805
Transcript:
RefSeq NM_001023563.3 (NON-CURRENT)
Match location:
Position 2389 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436570)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGTCTGGAAACTTACTCAATG
Hairpin Sequence:
5'-CCGG-GGTCTGGAAACTTACTCAATG-CTCGAG-CATTGAGTAAGTTTCCAGACC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGTCTGGAAACTTACTCAATGCTCGAGCATTGAGTAAGTTTCCAGACCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGTCTGGAAACTTACTCAATGCTCGAGCATTGAGTAAGTTTCCAGACC-3'

Other clones with same target sequence:

(none)