Construct: shRNA TRCN0000436578

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152237.1-220s21c1
DNA Barcode:
ATTTCGCTCCAGTGAGGCCTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAS2L1 (10634)
Gene Description:
growth arrest specific 2 like 1
Transcript:
RefSeq NM_152237.1 (NON-CURRENT)
Match location:
Position 220 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436578)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATTTCGCTCCAGTGAGGCCTA
Hairpin Sequence:
5'-CCGG-ATTTCGCTCCAGTGAGGCCTA-CTCGAG-TAGGCCTCACTGGAGCGAAAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATTTCGCTCCAGTGAGGCCTACTCGAGTAGGCCTCACTGGAGCGAAATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATTTCGCTCCAGTGAGGCCTACTCGAGTAGGCCTCACTGGAGCGAAAT-3'

Other clones with same target sequence:

(none)