Construct: shRNA TRCN0000436594

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153646.2-1822s21c1
DNA Barcode:
ATGTGCCGGGAAGACGATTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC24A4 (123041)
Gene Description:
solute carrier family 24 member 4
Transcript:
RefSeq NM_153646.2 (NON-CURRENT)
Match location:
Position 1822 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436594)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTGCCGGGAAGACGATTAG
Hairpin Sequence:
5'-CCGG-ATGTGCCGGGAAGACGATTAG-CTCGAG-CTAATCGTCTTCCCGGCACAT-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTGCCGGGAAGACGATTAGCTCGAGCTAATCGTCTTCCCGGCACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTGCCGGGAAGACGATTAGCTCGAGCTAATCGTCTTCCCGGCACAT-3'

Other clones with same target sequence:

(none)