Construct: shRNA TRCN0000436619

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003137.3-2368s21c1
DNA Barcode:
CCCATTAGGACATCCTTTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SRPK1 (6732)
Gene Description:
SRSF protein kinase 1
Transcript:
RefSeq NM_003137.3 (NON-CURRENT)
Match location:
Position 2368 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436619)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATTAGGACATCCTTTAAA
Hairpin Sequence:
5'-CCGG-CCCATTAGGACATCCTTTAAA-CTCGAG-TTTAAAGGATGTCCTAATGGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATTAGGACATCCTTTAAACTCGAGTTTAAAGGATGTCCTAATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATTAGGACATCCTTTAAACTCGAGTTTAAAGGATGTCCTAATGGG-3'

Other clones with same target sequence:

(none)