Construct: shRNA TRCN0000436645

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011890.4-1065s21c1
DNA Barcode:
GCGTTTGCCATAACTTGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sgcb (24051)
Gene Description:
sarcoglycan, beta (dystrophin-associated glycoprotein)
Transcript:
RefSeq NM_011890.4 (NON-CURRENT)
Match location:
Position 1065 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436645)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTTTGCCATAACTTGTAAT
Hairpin Sequence:
5'-CCGG-GCGTTTGCCATAACTTGTAAT-CTCGAG-ATTACAAGTTATGGCAAACGC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTTTGCCATAACTTGTAATCTCGAGATTACAAGTTATGGCAAACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTTTGCCATAACTTGTAATCTCGAGATTACAAGTTATGGCAAACGC-3'

Other clones with same target sequence:

(none)