Construct: shRNA TRCN0000436645
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_011890.4-1065s21c1
- DNA Barcode:
- GCGTTTGCCATAACTTGTAAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Sgcb (24051)
- Gene Description:
- sarcoglycan, beta (dystrophin-associated glycoprotein)
- Transcript:
- RefSeq NM_011890.4 (NON-CURRENT)
- Match location:
- Position 1065 (3UTR)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000436645)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCGTTTGCCATAACTTGTAAT
- Hairpin Sequence:
-
5'-CCGG-GCGTTTGCCATAACTTGTAAT-CTCGAG-ATTACAAGTTATGGCAAACGC-TTTTTTGAAT-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCGTTTGCCATAACTTGTAATCTCGAGATTACAAGTTATGGCAAACGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCGTTTGCCATAACTTGTAATCTCGAGATTACAAGTTATGGCAAACGC-3'
Other clones with same target sequence:
(none)
