Construct: shRNA TRCN0000436715

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178712.3-1839s21c1
DNA Barcode:
CTGATGGCTGTTCCGTCAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgrg2 (237175)
Gene Description:
adhesion G protein-coupled receptor G2
Transcript:
RefSeq NM_178712.3 (NON-CURRENT)
Match location:
Position 1839 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436715)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGATGGCTGTTCCGTCAAAG
Hairpin Sequence:
5'-CCGG-CTGATGGCTGTTCCGTCAAAG-CTCGAG-CTTTGACGGAACAGCCATCAG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGATGGCTGTTCCGTCAAAGCTCGAGCTTTGACGGAACAGCCATCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGATGGCTGTTCCGTCAAAGCTCGAGCTTTGACGGAACAGCCATCAG-3'

Other clones with same target sequence:

(none)