Construct: shRNA TRCN0000436721

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006770.3-1568s21c1
DNA Barcode:
GGCAGATCTGGCTGGATAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MARCO (8685)
Gene Description:
macrophage receptor with collagenous structure
Transcript:
RefSeq NM_006770.3 (CURRENT)
Match location:
Position 1568 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436721)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCAGATCTGGCTGGATAATG
Hairpin Sequence:
5'-CCGG-GGCAGATCTGGCTGGATAATG-CTCGAG-CATTATCCAGCCAGATCTGCC-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCAGATCTGGCTGGATAATGCTCGAGCATTATCCAGCCAGATCTGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCAGATCTGGCTGGATAATGCTCGAGCATTATCCAGCCAGATCTGCC-3'

Other clones with same target sequence:

(none)