Construct: shRNA TRCN0000436759

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018023.4-4706s21c1
DNA Barcode:
CCAGCGTTTCTGCAGTCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
YEATS2 (55689)
Gene Description:
YEATS domain containing 2
Transcript:
RefSeq NM_018023.4 (NON-CURRENT)
Match location:
Position 4706 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000436759)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCGTTTCTGCAGTCTTTG
Hairpin Sequence:
5'-CCGG-CCAGCGTTTCTGCAGTCTTTG-CTCGAG-CAAAGACTGCAGAAACGCTGG-TTTTTTGAAT-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCGTTTCTGCAGTCTTTGCTCGAGCAAAGACTGCAGAAACGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCGTTTCTGCAGTCTTTGCTCGAGCAAAGACTGCAGAAACGCTGG-3'

Other clones with same target sequence:

(none)